[Primer]
Primer pickup tool
Primer pickup tool
Example sequence
TAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGAC
Symplus Primer v0.1(beta)
1)
SEQUENCE_INCLUDED_REGION
A sub-region of the given sequence in which to pick primers. For example, often the first dozen or so bases of a sequence are vector, and should be excluded from consideration.
A sub-region of the given sequence in which to pick primers. For example, often the first dozen or so bases of a sequence are vector, and should be excluded from consideration.
2)
SEQUENCE_EXCLUDED_REGION
Primers and oligos may not overlap any region specified in this tag.
Primers and oligos may not overlap any region specified in this tag.
3)
SEQUENCE_TARGET
If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism.
If one or more Targets is specified then a legal primer pair must flank at least one of them. A Target might be a simple sequence repeat site (for example a CA repeat) or a single-base-pair polymorphism.
4)
PRIMER_NUM_RETURN
The maximum number of primer (pairs) to return. Primer pairs returned are sorted by their "quality", in other words by the value of the objective function (where a lower number indicates a better primer pair). Caution: setting this parameter to a large value will increase running time.
The maximum number of primer (pairs) to return. Primer pairs returned are sorted by their "quality", in other words by the value of the objective function (where a lower number indicates a better primer pair). Caution: setting this parameter to a large value will increase running time.
5)
PRIMER_MAX_NS_ACCEPTED
Maximum number of unknown bases (N) allowable in any primer.
Maximum number of unknown bases (N) allowable in any primer.
6)
PRIMER_PRODUCT_SIZE_RANGE
The associated values specify the lengths of the product that the user wants the primers to create. Format: from(bp)-to(bp, seperated by whitespace if any
The associated values specify the lengths of the product that the user wants the primers to create. Format: from(bp)-to(bp, seperated by whitespace if any
Except where otherwise noted, content on this wiki is licensed under the following license:CC Attribution-Noncommercial-Share Alike 3.0 Unported